1 6-Channcel High Throughput Octer Systems: Octet RH16 System

High Throughput Platform for Biologics Development

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Bio-Layer Interferometry (BLI) based on fiber optic biosensors
Sample volume
40–80 μL/well (384-well tilted bottom microplate) and 180–220 μL/well (96-well microplate), nondestructive testing, easily recovered
Data presentation
Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts
Robot compatible microplate and biosensor tray loading
Orbital flow capacity
Static or 100–1500 rpm
Analysis temperature
Ambient + 4°C to 40°C, in 1°C increments
Association rate constant (ka)
101 to 107 M-1 s-1
Dissociation rate constant (kd)
10-6 to 10-1 s-1
Affinity constant (KD)
1 mM to 10 pM
Baseline noise (RMS)
≤ 4 pm (RMS)
Baseline drift
< 0.1 nm/hour
Quantitation range
8–16 biosensors: 0.05–300 μg/mL of hIgG at 1000 rpm**; 0.5–2000 μg/mL at 400 rpm
Quantitation precision
CV <10%
150 lb (68.2 Kg)
Dimensions (H x W x D)
30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D)
Sample type
Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates
Sample plate
Standard, 96-well and 384-well, black, tilted bottom microplates
Molecular weight detection
>150 Da (8–16 biosensors)

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